DOT Urine Specimen Collector Training
- screenings4u

- Nov 10, 2025
- 3 min read
Updated: 5 days ago

DOT Urine Specimen Collector Training
Are you looking to advance your career in the drug testing industry? Becoming a certified DOT (Department of Transportation) urine specimen collector can open doors to new opportunities, whether you're seeking to enhance your current role or start your own business. At screenings4u, we offer comprehensive DOT and Non-DOT drug testing collector training that equips participants with the essential knowledge and skills necessary to collect, handle, and manage urine samples efficiently and professionally.
What You Will Learn
Our robust curriculum is designed to provide a thorough understanding of drug testing protocols and to ensure compliance with the Department of Transportation’s regulations in 49 CFR Part 40. Here’s a sneak peek of what you’ll learn:
1. DOT Regulated Agencies: Understand the various agencies that fall under DOT regulations and their safety-sensitive positions.
2. Collector Requirements: Explore the qualifications necessary to become a collector, including training responsibilities and restrictions.
3. Collection Site Security: Learn about the requirements for a secure collection site and the supplies needed.
4. Chain of Custody Form Completion: Master the proper steps required to fill out the Custody & Control Form (CCF) and distribute it correctly.
5. Urine Specimen Collection Process: Get trained on the entire collection process, including donor instructions, specimen inspection, and shipping protocols.
6. Monitored and Direct Observation Collections: Dive into the nuances of monitored collections and when direct observation is appropriate.
Course Curriculum
Our comprehensive training includes multiple units covering crucial aspects of drug testing collections:
Unit 1: Introduction & Background
Unit 2: The Collector
Unit 3: Collection Site & Supplies
Unit 4: Custody & Control Form (CCF)
Unit 5: The Collection Process
Unit 6: Monitored and Direct Observation Collections
Unit 7: Shy Bladder Protocol
Unit 8: Temperature Out of Range, Adulterated, and Substituted Specimens
Unit 9: Unusual Collections
Unit 10: Non-Regulated Drug Testing
Each unit aims to equip you with both theoretical knowledge and practical skills necessary for effective specimen collection.
Course Features
When you enroll in our training, you'll enjoy several benefits:
DOT Training: A thorough three-hour training session.
Mock Collections: Gain hands-on experience with five online video mock collections.
Digital Downloads: Access to downloadable training materials and relevant forms.
Certification: Upon completing the course and performing five error-free mock collections, you'll receive a completion certificate.
Expand Your Career or Start a Business
Completing this program not only enhances your knowledge but also opens up various career opportunities within the DOT drug testing collection field. This certification can serve as a stepping stone toward a fulfilling career, whether you aim to advance within your current profession or venture into entrepreneurship.
By partnering with screenings4u, we also help you set up your lab and Medical Review Officer (MRO) accounts. We facilitate the delivery of collection supplies directly to you, enabling you to start a successful drug testing business with discounted pricing on DOT and Non-DOT tests.
Trainer the Trainer Program
Looking to share your knowledge with others? Our Trainer the Trainer program equips experienced professionals with the skills necessary to train new collectors in proper urine specimen collection procedures, ensuring compliance with DOT regulations and the highest standards of quality and integrity in drug testing.
Training Options
We offer flexible training options tailored to your needs:
DOT Specimen Collector Training: $285.
DOT Specimen Collector Training + Hair Testing: $335, includes additional hair drug test training.
DOT Collector Train-the-Trainer: $525, for those looking to become trainers in the industry.
Join us at screenings4u today and take the first step toward becoming a certified DOT urine specimen collector. Expand your professional horizons and make a significant impact in the drug testing industry!



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